Cloning and expression of chitinase A, B, and C (chiA, chiB, chiC) genes from Serratia marcescens originating from Helicoverpa armigera and determining their activities
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چکیده
* Correspondence: [email protected]
منابع مشابه
Multiple genes involved in chitin degradation from the marine bacterium Pseudoalteromonas sp. strain S91.
A cluster of three closely linked chitinase genes organized in the order chiA, chiB and chiC, with the same transcriptional direction, and two unlinked genes, chiP and chiQ, involved in chitin degradation in Pseudoalteromnas sp. strain S91 were cloned, sequenced and characterized. The deduced amino acid sequences revealed that ChiA, ChiB and ChiC exhibited similarities to chitinases belonging t...
متن کاملJcb_201404127 1..12
Targeting of proteins to their sites of physiological function is an essential process in all cellular systems. Secretion of specific proteins completely out of the cell is a key process that bacteria use to adapt to and modulate their immediate environment (Trost et al., 2005). Six protein targeting and secretion systems have been identified in gram-negative bacteria, named type 1–type 6 (Econ...
متن کاملComparative studies of chitinases A and B from Serratia marcescens.
Serratia marcescens produces several chitinolytic enzymes, including chitinase A (ChiA) and chitinase B (ChiB). In this study, ChiB was purified to homogeneity using a newly developed protocol based on hydrophobic interaction chromatography. Subsequently, characteristics of ChiB and of the hitherto only partly characterized ChiA were determined and compared. Pure ChiA and ChiB shared several ch...
متن کاملIsolation and characterization of genes encoding two chitinase enzymes from Serratia marcescens.
Analysis of clones isolated from a cosmid DNA library indicates that the Serratia marcescens chromosome contains at least two genes, chiA and chiB, which encode distinct secreted forms of the enzyme chitinase. These genes have been characterized by inspection of chitinase activity and secreted proteins in Escherichia coli strains containing subclones of these cosmids. The two chitinase genes sh...
متن کاملA holin and an endopeptidase are essential for chitinolytic protein secretion in Serratia marcescens
Pathogenic bacteria adapt to their environment and manipulate the biochemistry of hosts by secretion of effector molecules. Serratia marcescens is an opportunistic pathogen associated with healthcare-acquired infections and is a prolific secretor of proteins, including three chitinases (ChiA, ChiB, and ChiC) and a chitin binding protein (Cbp21). In this work, genetic, biochemical, and proteomic...
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تاریخ انتشار 2015